MOLECULAR IDENTIFICATION OF PARASITIC MISTLETOE ARCEUTHOBIUM OXYCEDRI) OF JUNIPER ECOSYSTEM FROM DISTRICT ZIARAT, BALOCHISTAN, PAKISTAN.
- Resource Type
- Article
- Authors
- SHAHID, DAWOOD; AHMED, NAZEER; AHMED, SHAHJAHAN SHABBIR; SANI, IMRAN ALI; SIDDIQUI, MUHAMMAD FAHEEM; ALI, ASIF; IRFAN, SHAZIA; LEGHARI, SAADULLAH; REHMAN, ASIF UR
- Source
- FUUAST Journal of Biology. Jun2021, Vol. 11 Issue 1, p9-16. 8p.
- Subject
- *MISTLETOES
*PARASITIC plants
*FOREST declines
*GENETIC barcoding
*DATA recorders & recording
- Language
- ISSN
- 2222-0356
DNA barcoding is very effective tool in discriminating plants. Parasitic plants are responsible for destruction of plant locality. Arceuthobium oxycedri is a major cause of decline of Juniper forest Ziarat. There was no record of molecular data regarding this species in NCBI and GENBANK. Three markers i.e. RbcL, ITS-2 and Mat-k are used globally to obtain molecular data. CTAB method is used to extract DNA of eight A.oxycedri leaves from different areas of Sasnamanna, Ziarat. RbcL PCR amplification is 100% while Mat-k and ITS-2 were not amplified. RbcL discriminated Arceuthobium oxycedri at genus level as there is no data available in GENBANK and BOLD. 11 SNPs and 3 gaps found when compare A.oxycedri with Arceuthobium azoricum. In this research we have found that RbcL is much better primer than Mat-k and ITS-2 for identification of Arceuthobium oxycedri. [ABSTRACT FROM AUTHOR]