Genetic and Phytochemical Analysis of Armillaria mellea by RAPD, ISSR, and HPLC.
- Resource Type
- Article
- Authors
- Zheng, Peihe; Meng, Xiangying; Yu, Zhenxiang; Wu, Yin; Bao, Yongli; Yu, Chunlei; Li, Yuxin
- Source
- Analytical Letters. Jul2009, Vol. 42 Issue 10, p1479-1494. 16p. 1 Black and White Photograph, 2 Charts, 5 Graphs.
- Subject
- *MOLECULAR genetics
*GENETIC polymorphisms
*PHYTOCHEMICALS
*ARMILLARIA mellea
*CLUSTER analysis (Statistics)
*HIGH performance liquid chromatography
*MOLECULAR phylogeny
*PLEOMORPHIC fungi
- Language
- ISSN
- 0003-2719
Molecular genetic and phytochemical methods were combined to investigate 17 Chinese strains of Armillaria mellea. Ten random amplified polymorphic DNA (RAPD) primers yielded 106 bands, of which 94 were polymorphic; 80 out of the 84 bands produced by nine inter-simple sequence repeats (ISSR) markers were polymorphic. Contents of water-soluble constituents of mycelia were characterized by high-performance liquid-chromatography-diode array detection (HPLC-DAD) analyses. Cluster analyses of the genetic and phytochemical variation revealed that Armillaria mellea exhibited four chemotypes and four clusters from phytochemical contents. Phylogenetic groupings displayed in tree plots calculated from the RAPD and ISSR data matrix correlated with the geographical origin of the fungi materials. Genetic profiles partially correlated with the chemotypes. Phytochemical contents mainly correlated with the strains. A method based on RAPD and HPLC is described to establish genetic and chemical quality control of Armillaria mellea simultaneously. Ten RAPD primers and a coefficient of >0.95 can be used in authentication of Armillaria mellea. The fingerprints obtained with HPLC consist of 22 common peaks within 45 min. This method could be readily utilized as a quality-control method for pharmaceutical-grade Armillaria mellea. [ABSTRACT FROM AUTHOR]