Three new steroidal glycosides ( 1 – 3 ) and a novel natural product 4 firstly obtained from a plant source, together with two known steroidal glycosides ( 5 – 6 ) have been isolated from the whole plant of Reineckia carnea . Their structures were determined by physicochemical properties and spectroscopic methods, and their cytotoxic activities against human 1299 tumor cells were evaluated by the MTT method. Compounds 4 , 5 and 6 exhibited cytotoxicity with IC 50 values of 50.3 μmol·L − 1 , 67.2 μmol·L − 1 and 61.8 μmol·L − 1 , while compounds 1 , 2 , and 3 showed no cytotoxicity with the cells. [ABSTRACT FROM AUTHOR]