Eucommia ulmoides gum (EUG) is an eco-friendly and biodegradable novel polymer having the characteristics of both rubber and plastic, which shows excellent potential for conductive gasket material. A flexible, lightweight, and biodegradable EUG-based/carbon fillers composite was prepared via the hot pressing. The obtained composite demonstrates the advantageous properties, including preferable mechanical performance, thermal stability, thermal and electrical conductivity. Notably, the electrical conductivity of the nanocomposite exhibits preferable stability under several physical and chemical damages. Moreover, the EP/r3.8-C19.2 film delivers the average electromagnetic interference shielding effectiveness (EMI SE) of 21.2 dB, which is further enhanced by increasing the carbon nanotubes (CNTs) content and film thickness. Considering the increased abuse of non-degradable polymers, this green, degradable, facile, and low-cost strategy opens an alternative route for conductive gaskets, facilitating the practical application of EUG. • Flexible conductive film is prepared by Eucommia ulmoides gum and carbon filler. • Preferable electrically conductive of films exhibit stability by various damages. • The films show enhanced EMI SE by increasing fillers content and film thickness. • The potential use of Eucommia ulmoides gum in conductive gasket is studied. [ABSTRACT FROM AUTHOR]