Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain.
- Resource Type
- Article
- Authors
- Wang, Hao; Mo, Yongfang; Liu, Wenbo; Niu, Chenxia; He, Qijie; Ren, Tongwei; Ouyang, Kang; Chen, Ying; Huang, Weijian; Wei, Zuzhang
- Source
- Archives of Virology. Feb2024, Vol. 169 Issue 2, p1-8. 8p.
- Subject
- Language
- ISSN
- 0304-8608
Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome. [ABSTRACT FROM AUTHOR]