Isolation and Identification of Catechin by a New Method from Food Efficiency Stimulating Plant Alhagi camelorum.
- Resource Type
- Article
- Authors
- Laghari, Abdul Hafeez; Memon, Shahabuddin; Nelofar, Aisha; Khan, Khalid Mohammad.
- Source
- Pakistan Journal of Scientific & Industrial Research Series A: Physical Sciences. May/Jun2017, Vol. 60 Issue 2 A, p59-66. 8p.
- Subject
- *CATECHIN
*EPICATECHIN
*ALHAGI maurorum
*HIGH performance liquid chromatography
*OXIDANT status
*MASS spectrometry
- Language
- ISSN
- 2221-6413
The present article describes a new developed method for the simultaneous determination of (+)-catechin (1) and (-)-epicatechin (2), separated via HPLC. The method has been validated and applied on the real samples and 1 has been detected in both aerial and root parts of Alhagi camelorum without any ambiguity of fake positive or negative presence of 1 or 2 by virtue of dual detection system of UV and Mass Spectrometry. The antioxidant capacity was also investigated and a linear correlation has been noticed between the antioxidant capacity and the catechin amount in A. camelorum extracts. [ABSTRACT FROM AUTHOR]