Aim: This study evaluates the relationship between the occurrence of diabetic neuropathy in diabetic individuals and the effect of vitamin D levels in them. This study also evaluates whether there is an association with specific subtypes of diabetic neuropathy. Study design: An analytical cross-sectional study Place and Duration: This study was conducted at Shaheed Mohtarma Benazir Bhutto Medical College Lyari Karachi from June 2021 to July 2022. Methodology: Overall, 84 type 2 diabetes mellitus individuals were involved in this research. These 84 patients were divided into 4 groups, each having an equal number of patients. These groups were patients (A) with severe pain in diabetic neuropathy, (B) with no pain neuropathy, (C) with no pain neuropathy but having neuropathic ulcers, and (D) without neuropathy. The clinical examination as well as the neurological examination was done for all patients. They also went under nerve conduction study. The level of vitamin D and complete blood count were evaluated in these groups. Results: Group A (with severe pain due to diabetic neuropathy) had 71% of patients who had lower levels of vitamin D. The level of vitamin D ranged from 5.2-41 nanograms/dl and the mean was 17.6. Group B (with no pain neuropathy) had 61% of patients who had lower levels of vitamin D. The level of vitamin D ranged from 6.4-36.5 nanograms/dl and the mean was 19. Group D (without neuropathy) had only 6% of patients who had vitamin D deficiency. A negative correlation was seen between the neuropathy score and vitamin D level. The lesser the level of vitamin D, the more the neuropathy score. Conclusion: Vitamin D levels were reported to be lesser in those individuals who had diabetic neuropathy, and significantly in those who had severe pain due to diabetic neuropathy. [ABSTRACT FROM AUTHOR]