Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp. W1.
- Resource Type
- Article
- Authors
- Ma, Fang; Shi, Sheng-Nan; Sun, Tie-Heng; Li, Ang; Zhou, Ji-Ti; Qu, Yuan-Yuan
- Source
- Applied Microbiology & Biotechnology. Jun2013, Vol. 97 Issue 11, p5097-5103. 7p.
- Subject
- *CATECHOL
*BIOTRANSFORMATION (Metabolism)
*BENZENE
*TOLUENE
*HYDROXYLATION
- Language
- ISSN
- 0175-7598
Phenol hydroxylase gene engineered microorganism (PH) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and H NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 μM/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation. [ABSTRACT FROM AUTHOR]