This article, published in the journal Molecular Horticulture, explores the role of cytokinin in the patterning of adventitious root apical meristem (adRAM) from leaf cuttings. The study focuses on Arabidopsis thaliana leaf cuttings and reveals that cytokinin is required for the tissue differentiation of the adRAM. The researchers conducted experiments using cytokinin inhibitors and mutants, and observed adverse effects on root regeneration. They also analyzed the expression patterns of auxin and cytokinin markers during adventitious root organogenesis, which indicated that auxin and cytokinin function antagonistically in different steps and cells during cell fate transition and differentiation. The findings suggest that cytokinin contributes to the elimination of the stem cell niche identity and tissue differentiation in the adRAM, ensuring its maturation. [Extracted from the article]