Objective: Fungal spores are known as one of the important airborne allergens in human beings in both an outdoor and indoor environment. They are prevalent in all climates and in every geographical area and contribute a major part of suspended bio-particulate matter of the air. In this research investigated to understand the spectrum of fungal diversity of Tehran to prepare fungal spore calendar and influence of metrological parameters and air pollution in seasonal variation. Material and Methods: By using two samplers, one was daily records Burkard 7- days spore trap during 2011-2016 in Tehran areas with high air pollution (North, South, West, East, center) and control zone with clear area was (Parand city near the Tehran) and using a volumetric petri plate sampler put on the consisting media culture subrodextros agar with chloramphenichol was used and each colony were counted respectively and studying by optical microscopy. Results: 32 fungal spore types were recorded during the survey period. In the present study, were identified as the peak seasons for different areas in Tehran. Aspergillus species was the main contributor to the total fungal load with %76.7 followed by Alternaria, cladosporium, Fusarium, candida, penicillium, yeasts, Trichotechium, Pesudoallescheria, strilehyphae, Auerobasidium, Acromonium, Ulocladium, Mucor, Rhizopus, Curvularia and Epicoccum percents of a fungal spore calendar. An attempt has also been made to assess the allergenicity of some of the fungal types recorded from the atmosphere of different in Tehran and control areas. Meanwhile the value of daily count of fungal spores was higher in April, September, November and January more than other months and the meteorological parameter that most influenced airborne spore concentrations was temperature and humidity significantly and positively in the case of dry-air spores and fungal spores concentrations were observed on air polluted days in Tehran significantly p=0.001 with respect to the control area. Conclusion: Seasonal climatic changes are very important factors in reducing or increasing suspended spores in air and detailed study in this field can get a spore calendar to each patient who has Immunodeficiency, lung disease, allergic and asthma diseases and others. [ABSTRACT FROM AUTHOR]