Effects of Glycosaminoglycan from Urechis unicinctus on ADP-Induced Platelet Calcium and Membrane Glycoprotein Expressions in Rats.
- Resource Type
- Article
- Authors
- Cui, Qingman; Zhao, Ziyue; Gao, Tong; Yuan, Chunying
- Source
- Acta Haematologica. 2021, Vol. 144 Issue 1, p44-47. 4p.
- Subject
- *BLOOD platelets
*MEMBRANE glycoproteins
*BLOOD platelet aggregation
*CALCIUM
*BLOOD platelet activation
- Language
- ISSN
- 0001-5792
Background: Previous studies had shown that glycosaminoglycan (GAG) from Urechis unicinctus exerts an obvious antiplatelet aggregation effect. Objective: This study aims to investigate the effects of GAG from Urechis unicinctus on ADP-induced platelet calcium and membrane glycoprotein expressions in rats. Methods: Fura-2/AM fluorescence probe was used to measure intracytosolic free-calcium concentration ([Ca2+]i) of platelets and calculate platelet calcium influx and release concentrations. Flow cytometry was used to detect the expressions of platelet membrane glycoproteins GPIIb/IIIa (PAC-1) and P-selectin (CD62P) in rats. Results: The results showed that the GAG from U. unicinctus significantly inhibited the release of platelet calcium (p < 0.01) and the expressions of platelet GPII b/IIIa and P-selectin (p < 0.01) induced by ADP in rats but had no significant effect on the influx of platelet calcium (p > 0.01). Conclusions: This study indicated that GAG may inhibit platelet activation and aggregation by reducing the release of Ca2+ and ADP-induced expression of platelet membrane glycoprotein in rats. [ABSTRACT FROM AUTHOR]