Wound infection is a common factor that delays wound healing. The diagnosis of wound infection should be based on clinical observations of the presence of classic signs of infection. There is also a role for microbiological investigation, for example, using semi-quantitative wound swabs, to help guide systemic treatment. However, there is still a debate about the most accurate method for taking a wound swab. This review explores the research evidence underpinning the use of semi-quantitative wound swabs, focusing on the validity and reliability of this method of assessment. [ABSTRACT FROM AUTHOR]