Toluene 4-monooxygenase (T4MO) is a four-component complex that catalyzes the regiospecific, NADH-dependent hydroxylation of toluene to yield p-cresol. The catalytic effector (T4moD) of this complex is a 102-residue protein devoid of metals or organic cofactors. It forms a complex with the diiron hydroxylase component (T4moH) that influences both the kinetics and regiospecificity of catalysis. Here, we report crystal structures for native T4moD and two engineered variants with either four (ΔN4-) or 10 (ΔN 10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9-Å resolution, respectively. The crystal structures have C-alpha root-mean-squared differences of less than 0.8 Å for the central core consisting of residues 11-98, showing that alterations of the N-terminal have little influence on the folded core of the protein. The central core has the same fold topology as observed in the NMR structures of T4moD, the methane monooxygenase effector protein (MmoB) from two methanotrophs, and the phenol hydroxylase effector protein (DmpM). However, the root-mean-squared differences between comparable C-alpha positions in the X-ray structures and the NMR structures vary from ∼1.8 Å to greater than 6 Å. The X-ray structures exhibit an estimated overall coordinate error from 0.095 (0.094) Å based on the R-value (R free) for the highest resolution ΔN4-T4moD structure to 0.211(0.196) Å for the native T4moD structure. Catalytic studies of the ΔN4-, ΔN7-, and ΔN10- variants of T4moD show statistically insignificant changes in kcat, KM, kcat/KM, and KI relative to the native protein. Moreover, there was no significant change in the regio specificity of toluene oxidation with any of the T4moD variants. The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the ∼33 residue N-terminal region for catalytic activity. [ABSTRACT FROM AUTHOR]