The anti-biofilm activity of hydrogen peroxide against Escherichia coli strain FL-Tbz isolated from a pharmaceutical water system.
- Resource Type
- Article
- Authors
- Farjami, Afsaneh; Jalilzadeh, Sina; Siahi-Shadbad, Mohammadreza; Lotfipour, Farzaneh
- Source
- Journal of Water & Health. Oct2022, Vol. 20 Issue 10, p1497-1505. 9p.
- Subject
- *HYDROGEN peroxide
*ESCHERICHIA coli
*INDUSTRIALISM
*DRUG factories
*MICROPLATES
- Language
- ISSN
- 1477-8920
Biofilms are considered a significant reason for the failure of disinfection strategies in industrial water systems due to their resistance to antimicrobial agents. This study is designed to investigate the anti-biofilm activity of hydrogen peroxide (H2O2) at combinations of temperatures and contact times. For this purpose, an in vitro microtiter plate (MTP)-based model system was used for biofilm formation using Escherichia coli (E. coli) strain FL-Tbz isolated from the water system of a pharmaceutical plant. To investigate the anti-biofilm activity of H2O2, it was added at different concentrations (2--7% v/v) to biofilms and incubated at different temperatures (20--60 °C) for 10--40 min to find effective conditions to eradicate biofilms. Maximum biofilms were formed when bacterial suspensions were incubated at 37 °C for 96 h. The rate of biofilm formation using an environmental isolate was higher than that of standard strain. H2O2 at concentrations of 6.25% (v/v) at temperatures of 40 °C incubated for 25 min significantly eradicated the biofilms. [ABSTRACT FROM AUTHOR]