4-Biphenylaldehyde and 9-anthraldehyde: two fluorescent substrates for determining P450 enzyme activities in rat and human.
- Resource Type
- Article
- Authors
- Marini, S.; Grasso, E.; Longo, V.; Puccini, P.; Riccardi, B.; Gervasi, P. Giovanni
- Source
- Xenobiotica. Jan2003, Vol. 33 Issue 1, p1. 11p.
- Subject
- *ALDEHYDES
*CYTOCHROME P-450
*LABORATORY mice
*CARBOXYLIC acids
- Language
- ISSN
- 0049-8254
Examines the fluorescent substrates 4-Biphenylaldehyde (4-BA) and 9-anthraldehyde (9-AA) for determining the cytochrome P450 (CYPs) enzymes in rat and human. Oxidation of both aldehydes by CYPs to flourescent carboxylic acids; Increase of oxidation rate of aldehydes from rat micr- somes pretreated with phenobarbital; Correlations between CYP3A-dependent 6β-testosterone hydroxylase and the oxidation of 4-BA.