Aims/Purpose: Endothelial cell migration is enhanced in pathological processes of the eye involving high glucose, such as diabetic retinopathy (DR). The microRNA miR‐205‐5p is identified as an important regulator of vasculogenesis and could slow down cell migration. The aim of this study is to understand the role of mir‐205‐5p in endothelial cell proliferation under high glucose (HG) conditions. Methods: HUVEC were seeded at a density of 5.0 × 104 cells/cm2. After 48 h, a wound was produced by scratching cell monolayers with a pipette tip. The cells were treated with different conditioned mediums from ARPE‐19 cells: control media, control media and miR‐205‐5p mimic, HG media and HG media and miR‐205‐5p. Images were taken at 0, 4, 8 and 24 h. The gap distance was analysed with Image J (migration n = 4 and viability n = 6). Results: HUVEC cells treated with HG media from ARPE‐19 cells close the gap faster than HUVEC cells treated with ARPE‐19 control media or ARPE‐19 control media with mimic miR‐205‐5p. The administration of mimic miR‐205‐5p in HG media significantly reduce migration of HUVEC cells. Besides, the presence of HG for 5 days reduces ARPE‐19 cells viability however miR‐205‐5p mimic protect them, increasing cell viability in control. Conclusions: Angiogenesis is mostly due to an enhancement of endothelial cell migration. That seems to be related to a reduction of miR‐205‐5p, released from RPE cells. When a miR‐205‐5p mimic was added in the medium, cell migration slowed down. [ABSTRACT FROM AUTHOR]