T-helper 1 immunoreaction influences survival in muscle-invasive bladder cancer: proof of concept.
- Resource Type
- Article
- Authors
- Ingels, Alexandre; Salas, Rafael E. Sanchez; Ravery, Vincent; Fromont-Hankard, Gaëlle; Validire, Pierre; Patard, Jean-Jacques; Pignot, Géraldine; Prapotnich, Dominique; Olivier, Fabien; Galiano, Marc; Barret, Eric; Rozet, Francois; Weber, Nina; Cathelineau, Xavier
- Source
- Ecancermedicalscience. 2014, Vol. 8 Issue 474-494, p1-10. 10p.
- Subject
- *TH1 cells
*BLADDER cancer treatment
*BLADDER cancer patients
*RECEIVER operating characteristic curves
*LYMPHOCYTES
*TUMOR treatment
BLADDER tumors
- Language
- ISSN
- 1754-6605
Objective: To define immunoscore in bladder cancer studying T helper 1 (Th1) immunoreaction. To define a cancer-specific survival model based on Th1 cells infiltration. Methods: A total of 252 patients underwent primary transurethral resection of bladder tumour at our Institution. A retrospective review of a selected cohort with pT1 and muscle-invasive bladder cancer (MIBC) lesions was performed. Pathology blocks were marked with CD3 and CD8 antibodies. Immune cells density in stromal reaction (SR) was measured on five distinct high-power field (HPF) by two dedicated uro-pathologist blinded for patients' evolution. Statistics: Student test or non-parametric Wilcoxon test as appropriate to compare means between two groups. Receiver operating characteristics (ROC) curve to define markers threshold. Cox model to assess survival's predictors. Results: Ten pT1 and 20 MIBC consecutive cases were analysed. Median follow-up was 33.4 months. Immunohistological analysis for pT1 lesions featured limited SR. For MIBC, the mean density of lymphocytes in the SR was of 105/HPF (CD3) and 86/HPF (CD8). Survivors harboured higher lymphocytes densities versus non survivors (CD3: p = 0.0319; CD8: p = 0.0279). CD3 (p = 0.034) and CD8 (p = 0.034) lymphocytes densities were independently associated with cancer-specific survival on Cox model analyses. The retrospective design and small size of cohorts are the study limitations. Conclusions: High CD3 and CD8 lymphocytes SR densities are associated with better cancer-specific survival for MIBC. Th1 reaction against the tumour seems to be protective for bladder cancer. Further evaluation is warranted. [ABSTRACT FROM AUTHOR]