Novel monoclonal antibodies for identification of multicentric Castleman's disease; Kaposi's sarcoma-associated herpesvirus-encoded vMIP-I and vMIP-II
- Resource Type
- Article
- Authors
- Nakano, Kazushi; Katano, Harutaka; Tadagaki, Kenjiro; Sato, Yuko; Ohsaki, Eriko; Mori, Yasuko; Yamanishi, Koichi; Ueda, Keiji
- Source
- Virology. Apr2012, Vol. 425 Issue 2, p95-102. 8p.
- Subject
- *CASTLEMAN'S disease
*KAPOSI'S sarcoma-associated herpesvirus
*MONOCLONAL antibodies
*CHEMOKINES
*MACROPHAGES
*LYTIC cycle
*CELL lines
- Language
- ISSN
- 0042-6822
Abstract: Recent studies have indicated that vMIP-I and vMIP-II play important roles in the pathogenesis of Kaposi''s sarcoma-associated herpesvirus (KSHV)-related diseases due to the effects of these proteins on vascularization. We developed monoclonal antibodies against KSHV-encoded viral macrophage inflammatory protein-I (vMIP-I) and vMIP-II to study these expression profiles and reveal the pathogenesis of KSHV-related diseases. The MAbs against vMIP-I and vMIP-II reacted to KSHV-infected cell lines after lytic induction. Both vMIP-I and the vMIP-II gene products were detected 24h post-induction with 12-O-tetradecanoylphorbol-13-acetate until 60h in the cytoplasm of primary effusion lymphoma cell lines. In clinical specimens, both vMIP-I and vMIP-II gene products were detected in the tissues of patients with multicentric Castleman''s disease. On the other hand, only vMIP-II was detected in a subset of Kaposi''s sarcoma. We concluded that these antibodies might be powerful tools to elucidate the pathogenesis of KSHV-related diseases. [Copyright &y& Elsevier]