Background: Stevia is a natural sweetener plant with zero calorie content which is used as an alternative source of sugar. Stevioside extract from stevia is considered to be 300-350 times sweeter than sugar. It is safe for use by both diabetics and hypo glycaemics due to its low glycaemic index. Stevia has some bitter aftertaste along with sweetness due to the presence of some essential oils, tannins and flavonoids. Methods: Field experiment was conducted at Eastern Block of Crop Research Farm, Department of Agronomy, Naini Agricultural Institute, Prayagraj during Zaid 2018. The experiment was carried out in randomized complete block design comprising of three N levels i.e. 50, 75 and 100 kg N/ha and inter row spacing of 30, 40 and 50 cm with 20 cm of plant to plant spacing. The study was carried out to find out response of nitrogen levels and spacing on stevia. Result: Maximum plant height (51.24 cm), leaf area (4596.99 cm²) was observed with treatment 100 kg N ha-1 at 50 cm x 20 cm spacing and leaf area index (7.30), total soluble solids (TSS) (18.73 °Brix) was noticed with treatment 100 kg N ha-1 at 30 cm x 20 cm spacing, whereas the maximum Leaf number (342.52) was recorded with treatment 100 kg N ha-1 at 40 cm x 20 cm spacing. The results of this experiment revealed that increased dose of nitrogen with narrow spacing gave maximum quality. [ABSTRACT FROM AUTHOR]