Efficiently avoiding predators is critical for animal survival. However, little is known about how the experience of predator attack affects behaviors in predator defense. Here, we caught mice by tail to simulate a predator attack. We found that the experienced mice accelerated the flight in response to the visual threaten cue. Single predator attack didn't induce anxiety but increased the activity of innate fear or learning related nucleus. The predator attack induced acceleration of flight was partly rescued when we used drug to block protein synthesis which is critical in the learning process. The experienced mice significantly reduced the focused exploration on the floor during the environment exploration, which might facilitate the discovery of predator. These results suggest that mice could learn from the experience of predator attack to optimize their behavioral pattern to detect the predator cue immediately and response intensely, and therefore increase the probability of survival. • The experience of a predator attack enhances looming-evoked flight behavior without changing the anxiety level. • A single predator attack activates the learning related nucleus such as hippocampus and LA. • The effect of a predator attack on looming-evoked flight behavior was eliminated when protein synthesis was inhibited. • The experience of a predator attack reduces the floor exploration behavior during environment exploration. [ABSTRACT FROM AUTHOR]