Introduction: H9 Avian influenza viruses are a threat to public health. In Morocco, the first outbreaks of the H9N2 influenza virus were reported in 2016, affecting different types of poultry production. This study aims to identify the potential existence of H9 avian influenza infections in farmworkers and habitants in Sidi Kacem province in Morocco. Methods: we conducted a cross-sectional study performed on sera samples collected between November 1, 2017 and May 30, 2018 on 36 farms in the province of Sidi Kacem, where there is traditional breeding of poultry. Results: a total of 185 sera were received and analyzed at the Avian Pathology Unit of the Hassan II Agronomic and Veterinary Institute, of which 62.2% (115/185) were positive for H9. Anti-H9 was found in all municipalities, with positivity rates ranging from 38.9% in the municipality of Bir Taleb to 100% in the municipality of Sidi Azouz. The mean age was 45.0 + 14.9. The positivity rate ranged from 66.7% among farmers to 52.5% among livestock keepers. It also ranged from 46.9% among those with primary school education to 77.8% among those who had attended only preschool level. This rate was 60.0% among those with secondary school level, 67.4% among those who had never attended school and 75.0% among those with high education level. Conclusion: this study highlighted the H9 low pathogenic avian influenza virus exposure among the rural population in Sidi Kacem province in Morocco. Therefore, there is a need to monitor this subtype as part of the influenza sentinel surveillance system. [ABSTRACT FROM AUTHOR]