Debottlenecking of an In Vitro Enzyme Cascade Using a Combined Model‐ and Experiment‐Based Approach.
- Resource Type
- Article
- Authors
- Siedentop, Regine; Dziennus, Marlon; Lütz, Stephan; Rosenthal, Katrin
- Source
- Chemie Ingenieur Technik (CIT). Apr2023, Vol. 95 Issue 4, p543-548. 6p.
- Subject
- *ENZYMES
*ISOMERASES
- Language
- ISSN
- 0009-286X
Designing a feasible in vitro enzyme cascade is a challenging task that is often preceded by optimization steps. Computational methods can increase the efficiency of the optimization process by providing a deeper insight into the system. In this study, bottlenecks of a farnesyl pyrophosphate‐producing in vitro enzyme cascade were determined by a combination of in silico modeling and wet‐lab experiments. ATP and the precursor‐isomerizing enzyme isopentenyl diphosphate δ‐isomerase were identified as limitations of the in vitro enzyme cascade. [ABSTRACT FROM AUTHOR]