Fast and Sensitive Analysis of Amino Acids Using Automated Pre-Column Derivatization.
- Resource Type
- Article
- Authors
- Kraft, Vadim; Jochems, Philipp; Schad, Gesa J.
- Source
- LC-GC Europe. Jul2018, Vol. 31 Issue 7, p395-396. 2p.
- Subject
- *AMINO acids
*CHEMICAL derivatives
*DERIVATIZATION
*CHEMICAL precursors
*CHEMICAL reactions
- Language
- ISSN
- 1471-6577
The article focuses on the reaction of o-phthaldialdehyde (OPA) with primary amines at basic conditions, which is one of the most established methods for the pre-column derivatization of amino acids. It refers to the increase in the polarity of the derivatized products with the addition of mercaptopropionic acid (MPA) to the reaction solution.