Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells.
- Resource Type
- Article
- Authors
- Khanna, May; Imasaki, Tsuyoshi; Chikwana, Vimbai M.; Perez-Miller, Samantha; Hunter, Gerald O.; Mosley, Amber; Takagi, Yuichiro; Hurley, Thomas D.
- Source
- Protein Expression & Purification. Aug2013, Vol. 90 Issue 2, p78-83. 6p.
- Subject
- *GENE expression
*GLYCOGEN synthases
*RECOMBINANT proteins
*PHOSPHORYLATION
*INSECTS
- Language
- ISSN
- 1046-5928
Highlights: [•] Human glycogen synthase 1 (hGYS1) is purified by co-expression with glycogenin. [•] Recombinant hGYS1 produced in insect Hi5 cells is functional. [•] Recombinant hGYS1 is highly phosphorylated. [Copyright &y& Elsevier]