The wax obtained from whole fern of Actiniopteris radiata (Sw.) Link was analyzed by gas chromatography-mass spectrometry. 37 compounds, out of 38, were identified with the combination of fatty acids, alkanes, triterpenoids and sterols. 9,12,15-octadecatrien-1-ol (18.7%), Hexa decanoic acid (10.8%), Hepatacosane (8.3%), α-amyrin (7.0%), β-sitosterol (6.9%) and n-Hexadecanoic acid (6.1%) were the major contents in the wax. Agar well diffusion and micro dilution broth methods were applied to find antibacterial activity against both Gram-positive and Gram-negative bacteria. The wax has shown activity against all selected bacteria, indicating the broad-spectrum antibiotic activity. The maximum activity was observed against S. aureus. [ABSTRACT FROM AUTHOR]