Defects Associated with Mitochondrial DNA Damage Can Be Mitigated by Increased Vacuolar pH in Saccharomyces cerevisiae.
- Resource Type
- Article
- Authors
- Garipler, Görkem; Dunn, Cory D.
- Source
- Genetics. May2013, Vol. 194 Issue 1, p285-12SI. 17p.
- Subject
- *MITOCHONDRIAL DNA
*DNA damage
*HYDROGEN-ion concentration
*YEAST research
*CELL proliferation
- Language
- ISSN
- 0016-6731
While searching for mutations that alleviate detrimental effects of mitochondrial DNA (mtDNA) damage, we found that disrupting vacuolar biogenesis permitted survival of a sensitized yeast background after mitochondrial genome loss. Furthermore, elevating vacuolar pH increases proliferation after mtDNA deletion and reverses the protein import defect of mitochondria lacking DNA. [ABSTRACT FROM AUTHOR]