An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics.
- Resource Type
- Article
- Authors
- Neary, Joanne M.; Powell, Amanda; Gordon, Lyndsey; Milne, Claire; Flett, Fiona; Wilkinson, Barrie; Smith, Cohn P.; Micklefield, Jason
- Source
- Microbiology (13500872). Mar2007, Vol. 153 Issue 3, p768-776. 9p. 3 Diagrams, 2 Graphs.
- Subject
- *STREPTOMYCES coelicolor
*STREPTOMYCETACEAE
*ANTIBIOTICS
*ANTI-infective agents
*MICROBIAL metabolites
*BACTERIAL proteins
*HYDROXYLATION
*PHOSPHORYLATION
- Language
- ISSN
- 1350-0872
The article reports on a study to determine the in vivo functions of the hasP and asnO gene products of Streptomyces coelicolor, and whether they play a role in the hydroxylation and subsequent phosphorylation of the asparaginyl residues in calcium-dependent antibiotics (CDA). Results clearly indicate that HasP and AsnO are essential for the hydroxylation and phosphorylation of the Asn residue with the CDA.