Introduction: In the rural population of South India, tooth loss affects 48.7% of people. In addition, there are now more patients with more than five congenitally missing adult teeth. This may cause a person's quality of life to decline and cause them to experience both physical and psychological discomfort. Materials and Methods: The current cross-sectional descriptive study was conducted over a period of month using a questionnaire-based survey (February 2022). Using G-POWER software version 3.1, the minimum sample size was calculated as 100 people, considering a study with an 80% power and a = 0.5. Results: Around 59.6% have answered right when asked about the source of dental stem cells (DSCs). 13.5 percentage have no idea about sourcing of stem cells, 6.7% state dental pulp stem cell, a very small population state periodontal tissue to be the source, while a minor group state it to be from apical papilla, <1% have no idea. Conclusion: Data of this study indicate that although there is typically good awareness regarding DSCs origins, uses, and clinical research best practises, there is not a comprehensive comprehension of these topics. The respondents have shown a quite favorable attitude toward improving their understanding of DSCs. [ABSTRACT FROM AUTHOR]