NKX2.1 run-on mutation associated to familial brain-lung-thyroid syndrome Western blot of transfected cells, while confirming the increased molecular weight of mutant NKX2.1, shows that it is expressed at levels lower than the WT, with WT/mutant ratio similar to that of the corresponding mRNAs (Figure 1F). DATA AVAILABILITY STATEMENT Data availability statement: Data supporting the findings described in this study are available upon request. [Extracted from the article]