Synthesis of δ-Aminolaevulinate Synthase in vitro Using Hepatic mRNA from Chick Embryos with Induced Porphyria.
- Resource Type
- Article
- Authors
- Brooker, John D.; May, Brian K.; Elliott, William H.
- Source
- European Journal of Biochemistry. 5/12/80, Vol. 106 Issue 1, p17-24. 8p.
- Subject
- *MESSENGER RNA
*CHICKS
*EMBRYOS
*PORPHYRIA
*ENZYMES
*PROTEINS
*LIVER diseases
- Language
- ISSN
- 0014-2956
Polyadenylated mRNA was isolated from chick embryo liver following induction of hepatic porphyria. The RNA was translated in vitro using a wheat germ cell-free system and δ-aminolaevulinate synthase was identified in the translation products by indirect immunoprecipitation. The enzyme was not apparent in the translation products of polyadenylated RNA from non-induced livers. The molecular weight of δ-aminolaevulinate synthase synthesised in vitro was 70000 and the protein was estimated to represent up to 5 % of total products synthesized in vitro. These data demonstrate for the first time that induction of chick embryo liver δ-aminolaevulinate synthase activity in hepatic porphyria correlates with a large increase in the translational capacity of isolated polyadenylated RNA for this enzyme and, together with preliminary cDNA · RNA hybridization studies, indicate that an increase in the level of 6-aminolaevulinate synthase mRNA is responsible. [ABSTRACT FROM AUTHOR]