Nontyphoidal Salmonella enterica serovars are the leading cause of foodborne hospitalization and death episodes in the United States. In a typical war. it is estimated that > 1 million Americans experience illness due to foodborne nonophoidal Salmonellosis. While the private industry and manufacturing rely heavil, on results of validations studies for inactivation and decontamination of this prevalent and opportunistic pathogen of public health concern, availability of such studies conducted in realistic en, ironments of manufacturing and processing is limited due to pathogenic nature of the organism. The current study investigated effects of elevated hydrostatic pressure and a bioactive product from grapefruit seed extract (C' itricidal 1.1) for elimination of nontyphoidal Salmonella serovars. Additionally, sensitivitY of an a, irulent strain of the bacterium (Salmone!!a LTZ) investigated relative to the pathogenic Sahnone//a serm ars. Our results show that the avirulent strain utilized in the current study, has similar sensitivity to the treatments compared to nontyphoidal pathogenic Salmonella seroiars. Thus, this non - pathogenic strain could be used interchangeabh for public health microbiology hurdle validation studies as a surrogate for pathogenic Sahnone//a serovars. Additionallb. ~e observed >5 log reduction of the pathogen (i.e. > 99.999%) due to treatments uith elevated hydrostatic pressure at 500 M Pa and at 300 M Pa with presence of Citricidal ni. Our result additionally exhibits that combination of mild hydrostatic pressure and bioactive compounds such as Ci tricidal Tjl could be utilized to ensure safe and efficacious elimination of this prevalent pathogen of public health concern from common commodities. [ABSTRACT FROM AUTHOR]