hBMP-2 and hTGF-β1 expressed in implanted BMSCs synergistically promote the repairing of segmental bone defects.
- Resource Type
- Article
- Authors
- Yin, Chenghui; Chen, Jianping; Chen, Zongxiong; Zeng, Zhaoxun; Qiu, Junqin
- Source
- Journal of Orthopaedic Science. Jul2015, Vol. 20 Issue 4, p717-727. 11p.
- Subject
- *GENE expression
*MESENCHYMAL stem cells
*BONE abnormalities
*ADENOVIRUSES
*ORTHOPEDICS
*THERAPEUTICS
- Language
- ISSN
- 0949-2658
Objective: To evaluate the effects of co-expressing hBMP-2 and hTGF-β1 in BMSCs (bone marrow-derived mesenchymal stem cells) on the repairing process of radial segmental defects in rats. Methods: BMSCs were infected with a high titer recombinant adenovirus carrying hTGF-βl and/or hBMP-2 genes. Expression of exogenous genes in BMSCs was confirmed by RT-PCR and ELISA assays. In vitro effects of exogenous genes were assessed by MTT and ALP activity tests. A left radial defect model was created using 120 SD rats. Genetically modified or unmodified BMSCs were implanted with collagen sponge scaffolds into the 5-mm radial defect. The bone repair process was systematically monitored and evaluated by X-ray examinations, gross anatomic examinations, histological analyses, and biomechanical tests. Results: Expression of hBMP-2 and hTGF-β1 showed synergistic effects on promoting BMSC proliferation and enhancing ALP activity in vitro. Bone repair assays showed that hBMP-2 and hTGF-β1 promoted the production of chondrocytes and osteoblasts. Implanted BMSCs transfected with both hBMP-2 and hTGF-β1 led to the best bone repair outcome. Conclusion: hBMP-2 and hTGF-β1 can synergistically improve the bone repair process. Our results suggest a potential clinical value of combining hBMP-2 and hTGF-β1 in repairing bone defects. [ABSTRACT FROM AUTHOR]