Our research supports the evidence that using a portable pedal machine for 60 min per day enables to reduce cardiometabolic risk factors (waist-to-height ratio, us-CRP, total cholesterol and LDL-cholesterol) and improves weekdays movement behaviours in normal-weight healthy tertiary employees. Objectives: The aim of this study was to evaluate the effects of a portable pedal machine intervention (60 minutes per working day) for 12 weeks on healthy tertiary employees' cardiometabolic risk factors. Methods: Anthropometric parameters, body composition, cardiometabolic/inflammatory markers, physical fitness, physical activity, and sedentary time measured before and after the intervention were compared between office healthy workers who used a portable pedal machine (INT, n = 17) and those who did not (CTRL, n = 15). Results: The INT group improved Δultrasensitive C-reactive protein (P = 0.008), Δtotal cholesterol (P = 0.028), and Δlight-density lipoprotein cholesterol (P = 0.048) compared with the CTRL group (Δ: T1–T0). The intervention reduced daily sitting time (P ≤ 0.01) and increased time spent at light intensity (P ≤ 0.01) and moderate-to-vigorous (P ≤ 0.01) physical activity compared with baseline values. Conclusions: These findings suggest that promoting physical activity during workdays can reduce the negative health effects of spending too much time sitting and inactive. [ABSTRACT FROM AUTHOR]