Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars.
- Resource Type
- Article
- Authors
- Kayihan, C.; Eyidogan, F.; Afsar, N.; Oktem, H.; Yucel, M.
- Source
- Biologia Plantarum. Dec2012, Vol. 56 Issue 4, p693-698. 6p.
- Subject
- *ANTIOXIDANTS
*BARLEY
*GENE expression
*SUPEROXIDE dismutase
*MANGANESE enzymes
*CULTIVARS
- Language
- ISSN
- 0006-3134
The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (−3 °C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (−7 °C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity. [ABSTRACT FROM AUTHOR]