The glycerol conversion using hydrogenolysis reaction find the challenge due to the regioisomer products that are 1,2-PDO and 1,3-PDO. Low-cost catalyst based on Mo/SiO2 was introduced to substitute noble metal catalyst. The catalyst Mo/SiO2 was prepared using impregnation method with concentrations of molybdenum 5, 10, 15% and calcination temperature 110, 300 and 500 °C. The catalysts were characterized using FTIR, XRD powder, and FTIR-Pyridine. The catalytic testing for hydrogenation reaction was evaluated using molecular hydrogen donor from formic acid. High concentration of molybdenum tends to form aggregate that transformed to crystalline MoO3. The rising crystalline phase in the catalyst influence to present both Brønsted and Lewis acid site. The glycerol conversion shows the constant results around 42.5%. Meanwhile, the selectivity to 1,3-PDO was quite low and dependent on Brønsted acid site. [ABSTRACT FROM AUTHOR]