BACKGROUND: The lack of awareness and knowledge of the critical care professional toward organ donation has been proven to be the major reason for low organ donation rate. Without any administrative policy for mandatory reporting potential donors to Organ Procurement Organization (OPO) in China, education and training plays an essential role in merging organ donation into the clinical practice in critical care units. METHODOLOGY: Separately both Organ Procurement Organization of First People’s Hospital of Kunming City (KMOPO) and the OPO of No.303 Hospital of Chinese Peopleʼs Liberation Army collaborated with the Donation and Transplantation Institute DTI in Spain to design training programs for the health care professionals in the regions. A diagnosis study was performed and the needs for trainings were identified. The Transplant Procurement Management courses TPM® were adapted and performed in both regions. This training program has been verified by the provincial continuous education program of health care professionals. TPM courses include practical sessions and simulations to enhance proactive learning. They develop all phases of organ donation as well as strategies for successful organ procurement. International experts work in conjunction with the local skilled experts to lead the curse and interpreting during lectures were provided. RESULTS: KMOPO and TPM®, implemented the regional training program since 2014, aiming at continuously enhancing the knowledge and skill of critical care professionals in organ donation. In total, there were 146 students trained in 3 years. 60 out of these were ICU specialist. The annual number of donation after citizens’ death performed in KMOPO per million per population (pmp) has been increasing, from 0.33 in 2014 to 1.31 in 2016, accounting 107 (78.10 %) deceased organ donors in the region. Meanwhile in Guangxi province there have been 2 regional TPM courses in collaboration with DTI and the 303 OPO. The number of participants was 91. Out of this 72(80%) were ICU specialist. The number of the deceased donors in pmp for the Guangxi region have increase from 1.6 in 2014 in 1.99 in 2016. Counting of a total of 296 (49.25%) organ deceased cases in this region in the study period. DISCUSSION: Through adequate education program the awareness, knowledge and skills of the health care professional in China proved to enhance better understanding of organ donation practice and empower the professional abilities to increase the number of the potential donor and consequently the number of organ available for transplantation. The similar methodology should be expanded in more OPO around China.