Defining the roles of serine palmitoyltransferase-interacting proteins in the regulation of sphingolipid homeostasis.
- Resource Type
- Theses
- Authors
- Kimberlin, Athen N.
- Source
- Dissertation Abstracts International; Dissertation Abstract International; 77-09B(E).
- Subject
- Plant sciences
Biochemistry
- Language
- English
Summary: Our research also demonstrates that SPT substrate specificity can be altered through point mutations in AtssSPT and AtLCB1, leading to the production of aberrant long-chain bases (LCBs). Alteration of SPT substrate specificity may function as another regulatory control point by altering SPT products through changes in the composition of SPT subunits. We also discuss a system utilizing a point mutation in AtLCB1 that can be used as a tool to better measure SPT activity in planta. Collectively our data point towards a complex and nuanced regulatory scheme for maintaining sphingolipid homeostasis in Arabidopsis thaliana..