Cloning and characterization of two novel Taenia solium antigenic proteins and applicability to the diagnosis and control of taeniasis/cysticercosis.
- Resource Type
- Theses
- Authors
- Mayta, Holger
- Source
- Dissertation Abstracts International; Dissertation Abstract International; 69-12B.
- Subject
- Biology, Molecular
Health Sciences, Public Health
Biology, Parasitology
- Language
- English
Summary: Based on the genomic sequence of the Tso31 protein a nested PCR assay was developed for the specific diagnosis of taeniasis due to T. solium using DNA directly extracted from fecal samples. Samples collected and preserved in a 2% potassium dichromate solution were washed and DNA was directly purified using the FastDNARTMSPINRTMKit for Soil. Under field conditions the assay had a 100% sensitivity and specificity. The nested PCR described here might be a useful tool for the early diagnosis and prevention of taeniasis/cysticercosis.