This study presents a noninvasive device capable of subdiffraction-limited imaging. The research involves the construction of a two-fiber optical tweezer system to manipulate the stable stationary or ordered motion of a biological cell lens within an optical trap. By controlling the outgoing optical field at the fiber tip, the device enables large-range scanning imaging of the biological cell lens, expanding the field of view for ultramicroscopic imaging. The experimental results demonstrate that the biological cell lens can achieve magnification of nanostructures with a resolution as low as 100 nm under white light microscopy. Notably, when observing the surface of digital video disk (DVD) disks, the imaging effect of the biological cell lens surpasses direct observation, providing magnification factors of 1.5–2 times at the center of the lens and 1.3–1.5 times at the edges. These findings highlight the superior imaging capabilities of the bio-lens. The device presented in this study offers a new approach and technical means for leveraging biological cell lens imaging applications. It circumvents issues of sample damage and contamination during imaging, while its simple structure and ease of operation make it a promising tool in this field.