In carnivorous fish feed, soybean meal was widely used as the alternative protein source to fish meal, but its negative influence on fish immunity and gut health limits its application. Yeast β-glucan is a well-documented immunostimulant and prebiotic, and carboxymethylglucan has higher potential bioactivity. The present work was to investigate and compare the effects of yeast β-glucan and carboxymethylglucan on the immunity and gut health of turbot Scophthalmus maximus(with initial weight of 7.6 g) fed a diet containing 400 g kg−1soybean meal. Yeast β-glucan and carboxymethylglucan was respectively supplemented at the dose of 2 g kg−1. Enteritis was observed in fish fed the soybean meal diet. Beta-glucan or carboxymethylglucan supplementation significantly increased serum lysozyme activity, and the concentrations of IgM, complement 3 and complement 4. These two additives significantly decreased the infiltration of leukocytes in the lamina propria and submucosa and the expression of inflammatory cytokine of il-8. Compared with β-glucan, the fish fed carboxymethylglucan showed higher growth performance, feed utilization, lysozyme activity, concentrations of IgM, complement 3 and complement 4 but lower enteritis severity and inflammatory cytokines expression (il-1β, il-8, tnf-a and tgf-β). The structure of intestinal autochthonous bacteria was also altered by dietary yeast β-glucan and carboxymethylglucan. In conclusion, the present work confirmed the beneficial influence of dietary yeast β-glucan and carboxymethylglucan on turbots suffering from soy enteropathy. And carboxymethylglucan showed better protective effects than β-glucan against soybean meal-induced intestinal inflammation on turbot.