In order to investigate the diversity of spotted fever group (SFG) Rickettsiainfection in hard ticks, ticks were harvested from the forest areas in Suifenhe city, along the Chinese–Russian border and conventional PCR was carried out using universal SFG Rickettsiaprimers targeting gltA and ompA genes to screen for their infection with SFG Rickettsiaorganisms. Results showed that of the 215 ticks belonging to Ixodes persulcatus, Haemaphysalis concinnaand Haemaphysalis japonicaWarburton, 1908 species, 138 (64.2%) were positive for SFG Rickettsia. Three species of SFG Rickettsiawere detected, Rickettsia raoultii, Rickettsia heilongjiangensisand Candidatus Rickettsia tarasevichiae. No co-infection with different species of SFG Rickettsiawas found in any individual tick among the three tick species. We detected more than one SFG Rickettsiaspecies in ticks from each of the three tick species with an overlapping distribution and potentially similar transmission cycles of SFG Rickettsiain the areas surveyed. Consequently, different pathogenic rickettsial species may be involved in human cases of rickettsiosis after a bite of the three above-mentioned tick species in that area Rickettsia