Purpose: Transperineal Ultrasound-Guided Prostate Biopsy (TPPB) is gaining widespread interest for prostate cancer diagnosis due to its lower rates of infection compared to its transrectal counterpart. Existing TPPB models are costly, do not simulate needle passage, and do not allow for tissue sampling. We have constructed a low-cost, easily replicable TPPB model that effectively simulates needle passage and tissue sampling. Herein, we describe model fabrication and assess trainees’ knowledge, confidence, and satisfaction related to TPPB.Methods: We created a low-cost TPPB model using store-bought supplies, including gelatin, psyllium powder, and cooked beets. Trainees were asked to perform TPPB using the model with expert demonstration and supervision. Participant knowledge, confidence, and satisfaction was assessed before and after the simulation exercise using an online survey.Results: Total cost per individual prostate model was $22.76. Fifteen medical students and residents participated in the TPPB simulation. 93% of participants “agreed” or “strongly agreed” that the model was effective for technical instruction; 80% of participants “strongly agreed” that the model was superior to verbal or pictorial methods for understanding TPPB. Lastly, 93% of participants “agreed” or “strongly agreed” on model verisimilitude to in situ TPPB.Conclusions: We describe an inexpensive and reproducible TPPB model that proved to be an effective learning tool for trainees. Future studies will focus on improving model fidelity and developing a TPPB curriculum for urology trainees using this novel TPPB model.