Purpose: A comprehensive investigation of various maximum voluntary contraction (MVC) positions to determine the optimal positions for vastus lateralis (VL), biceps femoris (BF), gastrocnemius lateralis (GL), and tibialis anterior (TA).Methods: Twelve participants performed total of seventeen MVC positions for major lower limb muscle groups (VL, BF, GL, and TA). Neuromuscular activities were recorded by surface electromyography. Signals were smoothed by root mean square (RMS). Each MVC level were expressed as a percentage of MVC (% MVC). Statistical differences were measured with a one-way repeated measures analysis of variance and a Tukey’s HSD (p < 0.05).Results: Optimal MVC positions were found as follows: (i) VL: the combination of knee extension at 70° and 90° flexed knee in sitting position; (ii) BF: the combination of knee flexion at 30°, 45°, and 60° flexed knee in prone position; (iii) GL: unipedal standing position; (iv) TA: the combination of dorsiflexion in sitting position and ankle neutral, in standing position and ankle 110°, and in standing position and ankle 70°.Conclusion: This study confirms that multiple positions were needed to elicit the maximal MVC values for VL, BF, and TA. For GL, single MVC position should be performed to elicit the maximal MVC.