BACKGROUND:: Coral hydroxyapatite bone graft has been widely used for repairing bone defect in clinic; however, bone graft compound with antitumor drug still needs to be further studied. OBJECTIVE:: To prepare a cisplatin-impregnated coral hydroxyapatite bone graft and to observe the effect on primary-cultured cell proliferation of giant cell tumor of bone. DESIGN, TIME AND SETTING:: A contrast study was performed at Institute of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA and Department of Cell Biology, Basic Medical College, Southern Medical University from June to November 2006. MATERIALS:: Coral hydroxyapatite bone graft was provided by Institute of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA; Cisplatin was provided by Qilu Pharmaceutical Factory, Jinan; samples of human giant cell tumor of bone were provided by Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA. METHODS:: The cisplatin was impregnated with coral hydroxyapatite bone graft following vacuum freeze-drying to prepare bone graft with anti-tumor activity. Thereafter, the compound bone graft was immersed in simulating body fluid to obtain leaching liquor at the 2, 4, 6 and 8 weeks. MTT method was used to detect the inhibitor effect of leaching liuquid on proliferation of giant cell tumor of bone. Cisplatin-impregnated coral hydroxyapatite bone graft was considered as the controls. MAIN OUTCOME MEASURES:: Proliferation-inhibition rate of tumor cells. RESULTS:: The proliferation-inhibition rates of cisplatin-impregnated coral hydroxyapatite bone graft leaching liquor were 67.9%, 48.3%, 34.57%, and 28.4% respectively at 2, 4, 6 and 8 weeks. In addition, coral hydroxyapatite bone graft leaching liquor had no inhibitor effect on giant cell tumor of bone. CONCLUSION:: The cisplatin-impregnated coral hydroxyapatite bone graft allows sustained drug release and maintains excellent inhibitory effect on human giant cell tumor of bone cells within 8 weeks in vitro.Lin J, Yang JC.Inhibitory effects of coral hydroxyapatite bone graft on giant cell tumor of bone.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(34): 6637-6640. [http://www.crter.cn http://en.zglckf.com]