In this study, an investigation was conducted into the cell separation process for the production of cell-cultured meat. Establishing efficient management factors and addressing the fundamental aspects of obtaining cells from tissues are crucial for sustaining protein production. The separation of cells within tissues is a critical element to ensure the quality and stability of cells during cell culture. Identifying and managing potential physical, chemical, and biological hazards that may arise during cell culture is essential for maintaining cell quality and stability. Through these efforts, developing a safe and efficient cell-cultured meat production protocol becomes imperative, ultimately enhancing the potential utilization of cell-cultured meat in the food industry. Therefore, in this study, we aim to provide guidelines for enhancing the efficiency of experiments and the reliability of results through exploration of protocols related to cell-cultured meat and important hygiene management factors.