Background: Multidrug-resistant pathogens such as carbapenemase-producing Enterobacterales (CPE) and vancomycin-resistant enterococci (VRE) are of concern in hospitals worldwide. Hydrogen peroxide sterilizer is one of the ‘no-touch’ disinfection systems. In this study, we aimed to confirm the efficacy of the activated ionized hydrogen peroxide (AIHP) system space sterilizer developed in South Korea. Methods: Eighty sheets contaminated with CPE and VRE were placed in each room. The AIHP space sterilizer (MUGYUN, SUNGSAM, Korea) was used for sterilization. After the intervention, the mediums were incubated at 37℃ for 24 h, and colony-forming units (CFUs) were analyzed. Results: The median number of strains on the sheets before sterilization was 2.64×106 CFUs (6.42 log10 inoculum). The median log 10 reduction was 7.12 and 6.91 for CPE and VRE, respectively, after the intervention. Conclusion: The AIHP space sterilizer developed in Korea was effective for the sterilization of VRE and CPE in the healthcare environment.