Due to the constant high meat consumption, the number of livestock raised increased resulting in an increase in livestock manure generation and difficulties in feed production. Forage crops are usually integrated in other cropping systems during fallow season for self-sufficiency in feed supply. Livestock manure is introduced to agricultural lands in forms of compost or liquid fertilizers. containing 50-90% of antibiotics administered to livestock are discharged into livestock excrement as residues. Veterinary antibiotics are overused, not only to treat and prevent animal diseases, but also to promote livestock growth. This overuse causes their accumulation in agricultural fields, generating a higher spread of antibiotic-resistant bacteria in soil ecosystems. However, little attention has been paid to how antibiotic residues loaded manure compost use in feed cultivation absorbed by feed crops, contributes to the overall problem of antibiotic transmission to livestock and humans through the food chain. Therefore, a greater health risk exposure of humans to antibiotics persistently present in the environment. This study aimed to investigate antibiotic absorption tendency and residual antibiotics presence in soil and feed crops alfalfa (Medicago sativa L.) and triticale (x Triticosecale Wittm.) following sulfonamides treatment and to evaluate their effect on growth and quality of feed crops.In this experiment, livestock compost treated with three kind of sulfonamides: sulfamethoxazole (SMZ), sulfamethoxazole (SMX) and sulfathiazole (STZ) in four different concentrations: 0 (Con), 50 (S50), 250 (S250) and 500 (S500) mg kg-1 was used as an organic amendment in soil. According to antibiotic analysis results, all three kinds of sulfonamides SMZ, SMX, and STZ showed to be concentration-dependent to antibiotics presence in soil. Residual antibiotics concentrations in soil decreased over time in all treatment with a wider decrease rate in higher concentrations. Antibiotic absorption rate and the antibiotic degradation rate differed according to the type of crops, but the tendency was the same. In both alfalfa and triticale, all three antibiotics were high in the order of soil > root > shoot. The higher antibiotic concentration is, the higher antibiotic residual concentration in crop tissue gets. However, the translocation rate of sulfonamides absorbed by the two crops and their bioaccumulation in edible parts was not high. Nutrient analysis results demonstrated a negative corelation between sulfonamide antibiotics concentration and nitrogen content, water-soluble vitamins such as ascorbic acid, D-pantothermic acid and pyridoxine hydrochloride in alfalfa. Therefore, the influence of sulfonamides treatment on nutritional value of feed crops was confirmed. On the other hand, thiamine hydrochloride showed an increasing tendency, which is explained to be a mechanism of resistance to sulfonamide toxic stress, mitigating damage to crop growth and development. Through the results of this study, we confirmed that sulfonamide antibiotics are transferred from contaminated soils and absorbed by plants through livestock compost, and that the amount of antibiotics accumulated in crops increases with every compost administration. This research is expected to be used as a basis for monitoring a quantitative evaluation of antibiotics in soil and feed crops, and to establish a more accurate acceptance criteria for antibiotics residue that helps with livestock health risk assessment.