Protective effect of a freshwater algae Spirogyra sp. against skin aging induced by ultraviolet-B irradiation
- Resource Type
- DISSERTATION/ THESIS
- 왕뢰 / Wang, Lei
- , 2016,
Spirogyra sp. a freshwater algae, was collected during Januray to March in Kongju, South Korea. The lyophilized sample was extracted by 70% ethanol and obtained the 70% ethanol crude extract (SPE). The free radical scavenging activities and protective effects against skin aging induced by UV-B irradiation of SPE were evaluated in vitro on HaCaT cell line. The results suggests that SPE have protective effects against skin aging. Then, SPE was fractionated by organic solvets based on polarity. The ethyl actate fraction (SPEE) was found to contain a higher phenolic content than the other fractions, so it was selected for futher studies. The protective effects of SPEE against skin aging induced by UV-B irradiation were evaluated in vitro on HaCaT cell line and in vivo on zebrafish model. Anti-aging compounds of SPEE were seperated by one step using preparative centrifugal partition chromatography (CPC). This study suggests that Spirogyra sp. was rich in phenolic content and have strong radical scavenging activities and anti-UV-B irradiation activity. It has the potential to be used as a promising ingredient in cosmetic industry.