Hydrogels are widely used in various fields such as agriculture, membranes for water purification and removal of metal ions, bio and chemical sensors, contact lens, wound dressing and drug delivery as well as in food packaging. Hydrogels can be developed with various materials including synthetic plastics and bio-polymers.Multicomponent bio hydrogel films composed of agar, alginate collagen, calcium chloride (CaCl2) as crosslinking agent, cellulose nanofiber (CNF), chemical reduced silver nanoparticles (AgNPs) and grape seed extract (GSE) were prepared and their physical properties such as tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), surface color and UV-vis transmittance were determined. The physical properties were improved in some respects by loading different components severally. Specially for the swelling ratio, the combination of agar, alginate and collagen was an efficient way to prepare bio hydrogel film that the SR of agar/ alginate/ collagen ternary blend hydrogel films was 2363% which is 6 times more than simplex agar film (361%), twice over agar/ alginate dual blend film (1217%), and triple of agar/ collagen composite film (717%). The Ca2+crosslinking film, CNF and AgNPs incorporated film exhibited higher thermal stability on different levels. The preliminary test for packaging of fresh potatoes with the prepared films revealed that the blend bio hydrogel films can be used as packaging with high water permeability and antifogging function for highly respiring fresh agriculture produce. And the GSE and AgNPs incorporated films showed strong antimicrobial activity that can be applied as an active food packaging system.