In twentieth Century, the characters of Chinese animated movies have been renowned for their unique national characteristics. But with the further development of the reform and opening up in the late twentieth Century and the transformation of the market economy, the creation of animated characters with typical national style gradually disappeared, followed by the random imitation and copying of the excellent animated characters of the United States and Japan. To this day, the creation of Chinese animation role still cannot get rid of such realistic problems that is the lack of national character. In order to study the Chinese twentieth Century feature animation as the object, in-depth excavation of each animated film main character creation background, the root of national culture, to create animated characters based on the theory of analysis of formation and defects of character shaping of role relationship ethnic expression, typical ethnic character and national image, feature trajectory law, the formation and development of the twentieth Century animated characters were systematically discussed. It is concluded that the national character shaping of Chinese animation characters in the twentieth Century generally reflects the spirit of the national age. It takes full advantage of the distinct national elements and combines with national culture and folk art under the influence of Chinese traditional culture. And you can find the role of concrete prototype reference, absorbs the essence of China national culture, shows the role a noble quality, two opposites of justice over evil and the relationship between the individual to maintain the national features of collectivism. The role of Chinese animated film in twentieth Century is worth the reference and thinking of the Chinese animation in the new century.